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ct226 flag 19.2 kda  (INCF)

 
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    INCF ct226 flag 19.2 kda
    (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or <t>CT226</t> FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.
    Ct226 Flag 19.2 Kda, supplied by INCF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ct226+flag+19%2E2+kda/c++trachomatis+l2+ct226+flag/bio_rxiv__616896-247-13-17
    Average 90 stars, based on 1 article reviews
    ct226 flag 19.2 kda - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells"

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells

    Journal: bioRxiv

    doi: 10.1101/616896

    (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or CT226 FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.
    Figure Legend Snippet: (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or CT226 FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.

    Techniques Used: Infection, Expressing, Staining, Immunofluorescence, Construct, Microscopy

    HeLa cells seeded in a 6-well plate with glass coverslips were infected with C. trachomatis L2 CT226 FLAG or IncF FLAG and either not induced or induced for expression at 7 hpi with 5 nM aTc (CT226 FLAG ) or 1 nM aTc (IncF FLAG ). (A) At 24 hpi, coverslips were removed, fixed in 4% paraformaldehyde, and stained to visualize FLAG (red), inclusion membrane marker (IncA; green), Chlamydiae (magenta), and DNA (blue). Coverslips were imaged using a Zeiss ApoTome.2 with 100x magnification. Scale bar = 10 µm. (B) The remaining cells were collected, solubilized, normalized, and affinity purified using FLAG beads. Clarified lysates (soluble) and eluates were probed for construct expression (FLAG; CT226 FLAG 19.2 kDa and IncF FLAG 11.3 kDa), and LRRF1 (dimer 160 kDa). See supplementary figure 5.
    Figure Legend Snippet: HeLa cells seeded in a 6-well plate with glass coverslips were infected with C. trachomatis L2 CT226 FLAG or IncF FLAG and either not induced or induced for expression at 7 hpi with 5 nM aTc (CT226 FLAG ) or 1 nM aTc (IncF FLAG ). (A) At 24 hpi, coverslips were removed, fixed in 4% paraformaldehyde, and stained to visualize FLAG (red), inclusion membrane marker (IncA; green), Chlamydiae (magenta), and DNA (blue). Coverslips were imaged using a Zeiss ApoTome.2 with 100x magnification. Scale bar = 10 µm. (B) The remaining cells were collected, solubilized, normalized, and affinity purified using FLAG beads. Clarified lysates (soluble) and eluates were probed for construct expression (FLAG; CT226 FLAG 19.2 kDa and IncF FLAG 11.3 kDa), and LRRF1 (dimer 160 kDa). See supplementary figure 5.

    Techniques Used: Infection, Expressing, Staining, Marker, Affinity Purification, Construct

    Proposed model of Inc organization based on mass spectrometry identified chlamydial Inc proteins using IncA-APEX2 and IncF-APEX2 proximity labeling constructs and bacterial two-hybrid assays (BACTH) to test protein-protein interactions. Based on these data we propose four possible scenarios for the spatial organization of Incs and how these Incs were detected using the APEX2 proximity labeling system: (1) IncF interacts with CT226 which binds LRRF1. (2) IncA interacts with CT226 which binds LRRF1. (3) IncA binds IncF and CT226 which binds LRRF1. (4) IncA, CT223, IncF, and CT226 (which binds LRRF1) all interact with each other. CT223 was statistically significant by SAINT analysis from mass spectrometry data and was able to interact with IncF and IncA by BACTH.
    Figure Legend Snippet: Proposed model of Inc organization based on mass spectrometry identified chlamydial Inc proteins using IncA-APEX2 and IncF-APEX2 proximity labeling constructs and bacterial two-hybrid assays (BACTH) to test protein-protein interactions. Based on these data we propose four possible scenarios for the spatial organization of Incs and how these Incs were detected using the APEX2 proximity labeling system: (1) IncF interacts with CT226 which binds LRRF1. (2) IncA interacts with CT226 which binds LRRF1. (3) IncA binds IncF and CT226 which binds LRRF1. (4) IncA, CT223, IncF, and CT226 (which binds LRRF1) all interact with each other. CT223 was statistically significant by SAINT analysis from mass spectrometry data and was able to interact with IncF and IncA by BACTH.

    Techniques Used: Mass Spectrometry, Labeling, Construct

    Related Articles

    Affinity Purification:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Construct:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Western Blot:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Infection:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Expressing:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Staining:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Immunofluorescence:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Microscopy:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Marker:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Mass Spectrometry:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Labeling:

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells
    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).



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    ct226 flag 19.2 kda  (INCF)
    90
    INCF ct226 flag 19.2 kda
    (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or <t>CT226</t> FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.
    Ct226 Flag 19.2 Kda, supplied by INCF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ct226+flag+19%2E2+kda/c++trachomatis+l2+ct226+flag/bio_rxiv__616896-247-13-17
    Average 90 stars, based on 1 article reviews
    ct226 flag 19.2 kda - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or CT226 FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.

    Journal: bioRxiv

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells

    doi: 10.1101/616896

    Figure Lengend Snippet: (A) Hela cells seeded on glass coverslips were infected with C. trachomatis L2 Inc-APEX2 transformants or CT226 FLAG transformants and induced for expression at 20 hpi (IncF-APEX2 was induced with 1 nM aTc; 5 nM aTc for all other transformants). At 24 hpi, coverslips were fixed with ice cold methanol and stained for immunofluorescence to visualize construct expression (FLAG) or CT223 (red), LRRF1 (green), Chlamydiae and DNA (DRAQ5 and MOMP; pink). Coverslips were imaged by Zeiss Elyra super-resolution microscopy 63×2x with structural illumination (SIM). Scale bar = 5 µm. (B) SIM 3D snapshot of C. trachomatis L2 CT226 FLAG infected HeLa cells with CT226 FLAG and LRRF1 positive fibers. (C) SIM 3D snapshot of C. trachomatis L2 IncA-APEX2 infected HeLa cells with IncA fibers. Arrows indicate co-localization between the indicated expressed construct and LRRF1.

    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Techniques: Infection, Expressing, Staining, Immunofluorescence, Construct, Microscopy

    HeLa cells seeded in a 6-well plate with glass coverslips were infected with C. trachomatis L2 CT226 FLAG or IncF FLAG and either not induced or induced for expression at 7 hpi with 5 nM aTc (CT226 FLAG ) or 1 nM aTc (IncF FLAG ). (A) At 24 hpi, coverslips were removed, fixed in 4% paraformaldehyde, and stained to visualize FLAG (red), inclusion membrane marker (IncA; green), Chlamydiae (magenta), and DNA (blue). Coverslips were imaged using a Zeiss ApoTome.2 with 100x magnification. Scale bar = 10 µm. (B) The remaining cells were collected, solubilized, normalized, and affinity purified using FLAG beads. Clarified lysates (soluble) and eluates were probed for construct expression (FLAG; CT226 FLAG 19.2 kDa and IncF FLAG 11.3 kDa), and LRRF1 (dimer 160 kDa). See supplementary figure 5.

    Journal: bioRxiv

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells

    doi: 10.1101/616896

    Figure Lengend Snippet: HeLa cells seeded in a 6-well plate with glass coverslips were infected with C. trachomatis L2 CT226 FLAG or IncF FLAG and either not induced or induced for expression at 7 hpi with 5 nM aTc (CT226 FLAG ) or 1 nM aTc (IncF FLAG ). (A) At 24 hpi, coverslips were removed, fixed in 4% paraformaldehyde, and stained to visualize FLAG (red), inclusion membrane marker (IncA; green), Chlamydiae (magenta), and DNA (blue). Coverslips were imaged using a Zeiss ApoTome.2 with 100x magnification. Scale bar = 10 µm. (B) The remaining cells were collected, solubilized, normalized, and affinity purified using FLAG beads. Clarified lysates (soluble) and eluates were probed for construct expression (FLAG; CT226 FLAG 19.2 kDa and IncF FLAG 11.3 kDa), and LRRF1 (dimer 160 kDa). See supplementary figure 5.

    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Techniques: Infection, Expressing, Staining, Marker, Affinity Purification, Construct

    Proposed model of Inc organization based on mass spectrometry identified chlamydial Inc proteins using IncA-APEX2 and IncF-APEX2 proximity labeling constructs and bacterial two-hybrid assays (BACTH) to test protein-protein interactions. Based on these data we propose four possible scenarios for the spatial organization of Incs and how these Incs were detected using the APEX2 proximity labeling system: (1) IncF interacts with CT226 which binds LRRF1. (2) IncA interacts with CT226 which binds LRRF1. (3) IncA binds IncF and CT226 which binds LRRF1. (4) IncA, CT223, IncF, and CT226 (which binds LRRF1) all interact with each other. CT223 was statistically significant by SAINT analysis from mass spectrometry data and was able to interact with IncF and IncA by BACTH.

    Journal: bioRxiv

    Article Title: Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells

    doi: 10.1101/616896

    Figure Lengend Snippet: Proposed model of Inc organization based on mass spectrometry identified chlamydial Inc proteins using IncA-APEX2 and IncF-APEX2 proximity labeling constructs and bacterial two-hybrid assays (BACTH) to test protein-protein interactions. Based on these data we propose four possible scenarios for the spatial organization of Incs and how these Incs were detected using the APEX2 proximity labeling system: (1) IncF interacts with CT226 which binds LRRF1. (2) IncA interacts with CT226 which binds LRRF1. (3) IncA binds IncF and CT226 which binds LRRF1. (4) IncA, CT223, IncF, and CT226 (which binds LRRF1) all interact with each other. CT223 was statistically significant by SAINT analysis from mass spectrometry data and was able to interact with IncF and IncA by BACTH.

    Article Snippet: The FLAG affinity purified constructs were also detected by western blot ( , CT226 FLAG 19.2 kDa, IncF FLAG 11.3 kDa (monomer) and 22.6 kDa (dimer); Fig S5).

    Techniques: Mass Spectrometry, Labeling, Construct